Journal: The FASEB Journal

Article Title: The role of chemerin in the regulation of cGAS‐STING pathway in gestational diabetes mellitus placenta

doi: 10.1096/fj.202201611r

Figure Lengend Snippet: FIGURE 1 The differences of placental morphology and mitochondrial structure in GDM patients and normal pregnant women. (A) Representative TEM image of GDM placenta mitochondria (scale bar = 500 nm). (B) Representative TEM image of placenta mitochondria in the control group (scale bar = 500 nm). (C) Specific surface of mitochondria analysis. (D) Representative image of HE staining in GDM placenta (scale bar = 100 μm). (E) Representative image of HE staining in placenta of the control group (scale bar = 100 μm). White arrows indicated placental mitochondria. GDM: gestational diabetes mellitus; TEM: transmission electron microscope; HE: hematoxylin eosin. Data were shown as mean ± SD (n ≥ 3). *p < .05.

Article Snippet: 3.7 | The mitochondrial membrane potential was diminished and phosphorylation of TBK1 and IRF3 protein were enhanced in the HTR- 8/ SVneo cell model To confirm the changes of the mitochondrial membrane potential in the HG and IR cell models, JC- 1 assay kit (C2006, Beyotime Biotechnology, China) was used according to the manufacturer's protocol.

Techniques: Control, Staining, Transmission Assay, Microscopy

Journal: Redox Biology

Article Title: 17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy

doi: 10.1016/j.redox.2023.102708

Figure Lengend Snippet: E 2 rescued primary cultured hippocampal neurons from erastin-induced ferroptosis. (A) Cell viability of primary cultured hippocampal neurons treated with or without erastin (20 μM), E 2 (30 μM) and Lip-1 (10 μM) for 12 h (n = 3). (B) Relative RNA level of DHODH (n = 3). (C) Representative images of western blots and quantitative analysis showing the expression levels of DHODH, GPX4, TfR, ferritin, FPN1, PCBP1 and GAPDH in primary cultured hippocampal neurons (n = 3). (D) and (F) Representative images of BODIPY C11 581/591 staining (scale bar = 50 μm) and quantitative analysis of lipid peroxidation in hippocampal neurons by the green/red fluorescence ratio. The cell nuclei were stained with DAPI (blue). (E) and (G) Representative images of MitoSOX staining (scale bar = 50 μm) and quantitative analysis of the red/blue fluorescence ratio. (H) and (I) MMP changes were measured by FCM analysis of JC-1 using annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cells were plated in precoated 6-well plates and incubated with 1 mL of working solution diluted from a Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Solarbio) for 20 min. After complete digestion, the cells were washed and subjected to flow cytometry (FCM) analysis.

Techniques: Cell Culture, Western Blot, Expressing, Staining, Fluorescence

Journal: Redox Biology

Article Title: 17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy

doi: 10.1016/j.redox.2023.102708

Figure Lengend Snippet: The ferroptosis resistance to erastin induced ferroptosis mediated by E 2 can be blocked by BQR in primary cultured hippocampal neurons. (A) Cell viability of primary cultured hippocampal neurons treated with or without erastin (20 μM), E 2 (30 μM) and BQR (5 μM) for 12 h (n = 3). (B) Relative RNA level of DHODH (n = 3). (C) Representative images of western blots and quantitative analysis showing the expression levels of DHODH, GPX4, TfR, ferritin, FPN1, PCBP1 and GAPDH in primary cultured hippocampal neurons (n = 3). (D) and (F) Representative images of BODIPY C11 581/591 staining (scale bar = 50 μm) and quantitative analysis of lipid peroxidation in hippocampal neurons by the green/red fluorescence ratio. The cell nuclei were stained with DAPI (blue). (E) and (G) Representative images of MitoSOX staining (scale bar = 50 μm) and quantitative analysis of the red/blue fluorescence ratio. (H) and (I) MMP changes were measured by FCM analysis of JC-1 using annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cells were plated in precoated 6-well plates and incubated with 1 mL of working solution diluted from a Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Solarbio) for 20 min. After complete digestion, the cells were washed and subjected to flow cytometry (FCM) analysis.

Techniques: Cell Culture, Western Blot, Expressing, Staining, Fluorescence

Journal: Redox Biology

Article Title: 17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy

doi: 10.1016/j.redox.2023.102708

Figure Lengend Snippet: The ferroptosis resistance to FAC induced ferroptosis mediated by E 2 can be blocked by BQR in primary cultured hippocampal neurons. (A) Cell viability of primary cultured hippocampal neurons treated with or without FAC (0.5 mg/L), E 2 (30 μM) and BQR (5 μM) for 12 h (n = 3). (B) Relative RNA level of DHODH (n = 3). (C) Representative images of western blots and quantitative analysis showing the expression levels of DHODH, GPX4, TfR, ferritin, FPN1, PCBP1 and GAPDH in primary cultured hippocampal neurons (n = 3). (D) and (G) Representative images of BODIPY C11 581/591 staining (scale bar = 50 μm) and quantitative analysis of lipid peroxidation in hippocampal neurons by the green/red fluorescence ratio. The cell nuclei were stained with DAPI (blue). (E) and (H) Representative images of MitoSOX staining (scale bar = 50 μm) and quantitative analysis of the red/blue fluorescence ratio. (F) and (I) MMP changes were measured by FCM analysis of JC-1 using annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cells were plated in precoated 6-well plates and incubated with 1 mL of working solution diluted from a Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Solarbio) for 20 min. After complete digestion, the cells were washed and subjected to flow cytometry (FCM) analysis.

Techniques: Cell Culture, Western Blot, Expressing, Staining, Fluorescence